Evaluation of a Positive Direct Antiglobulin Test

This presentation is from my time at National Guard Health Affairs Riyadh and suggests an algorithm to assess the clinical significance of the reaction. In my opinion, the essence of immunohematology testing is the antiglobulin test so I would spend much time with technical and medical staff, including trainees/fellows (even those not based in transfusion medicine) to make certain they understood how to interpret it.

If it is a neonate and the mother is ABO-incompatible, always test against reagent A1 and B cells.