I periodically repost this panel because it is so important not to miss an anti-Jka since it may cause severe hemolysis. Routinely, I use gamma-heavy-chain-specific antiglobulin AHG reagent; but to the best of my knowledge, I have not missed an anti-Jka. In this case, there were nonspecific reactions in the panels—many panels were performed with inconclusive results. As a last resort, I used a polyspecific AHG reagent and found the antibody. I have only encountered two such antibodies in many years of practice in the Middle East.
My advice: if you have only nonspecific reactions with multiple panels and suspect hemolysis, I would do a polyspecific AHG panel to look for this. Be sure to include HOMOZYGOUS Jka+ and Jkb+ cells.
I want to thank Mr. Stephen Fuentes, SBB(ASCP) for his handling of this workup.