This is a repost of this rare antibody with a few new additional comments.
This is a rare anti-Jka antibody that only reacts with polyspecific AHG and only in homozygous Jka+ cell. In the past three decades, I have only seen three of these.
Almost all anti-Jka and anti-Jkb antibodies can be detected using heavy-chain gamma specific AHG. I prefer to use this reagent since it minimizes interference from cold antibodies.
Whenever you have a nonspecific antibody, never discount the possibility of a Kidd antibody. Consider doing extended phenotyping and include Jka and Jkb typing and always check for dosage effect. I have a specific post on my approach to nonspecific antibodies.
Select antibody screening cells including homozygous Jka+ and homozygous Jkb+. This usually requires a three-cell screen.
Never forget that a negative result only means not-detected. If there is other evidence for hemolysis, consider performing additional studies.