DAT-Negative Hemolytic Anemias

This is a summary diagram for the causes of DAT-negative hemolytic anemia, both immune and non-immune. For immune cases, a negative DAT may indicate “not-detected” with the amount of immunoglobulin on the RBC being below the threshold of the test methodology being used. Also rare IgA associated cases will not be detected unless a specific alpha-heavy chain monospecific reagent is used.