Principle:
Immediate-spin crossmatch ISXM is an abbreviated compatibility testing that detects most ABO incompatibility. AABB Standards restricts its use to specific situations as indicated in the Policy section below.
Policy:
- Applicability:
- ISXM may only be used when ALL of the following conditions are met:
- Negative antibody screen
- No history of antibodies
- No ABO/D antigen typing discrepancies
- Less than two (2) ABO/D determinations are on-file.
- Use the computer/electronic crossmatch whenever its specific criteria are met.
- ISXM may only be used when ALL of the following conditions are met:
- Testing:
- ISXM consists of reacting an RBC suspension with patient/serum or plasma, centrifuging, and immediately reading for agglutination or hemolysis. THERE IS NO INCUBATION STEP, NO USE OF ANTIHUMAN GLOBULIN AHG REAGENT.
- If any hemolysis or agglutination is detected, the crossmatch is incompatible:
- Repeat the ISXM.
- Repeat the patient ABO/D AND unit ABO/D typings.
- Repeat the antibody screen.
- Perform full AHG crossmatch AND AHG/enzyme antibody panels.
- Refer the case to senior technical staff or a Transfusion Medicine consultant for further instructions.
- Case review will be documented in Medinfo HIIG computer system.
- DO NOT RELEASE THE RBCS UNTIL SO INSTRUCTED BY SENIOR STAFF.
References:
- Technical Manual, Current Edition, AABB, Bethesda, MD, USA
- Standards for Blood Banks and Transfusion Services, Current Edition, AABB, Bethesda, MD, USA
- Guidelines to the Preparation, Use, and Quality Assurance of Blood Components, European Committee (Partial Agreement) on Blood Transfusion (CD-P-TS), Current Edition